Antimicrobial susceptibility of fructophilic lactic acid bacteria on phzM gene of pseudomonas aeruginosa isolates from wounds infected
DOI:
https://doi.org/10.2478/AMB-2024-0078Keywords:
P. aeruginosa, phzM gene, bee gut, MDRAbstract
Introduction: The study was to isolate and characterize fructophilic lactic acid bacteria (FLAB) species from the honey bee gut. Based on the results of this study, it was found that the FLAB species obtained from honey were gram-positive and catalase-negative, and this identification was confirmed through 16S rRNA gene sequencing. Materials and methods: The results indicated that yeast extract was the most effective nitrogen source, while glucose was the preferred carbon source for cell-free supernatant (CFS) production. The optimal pH for CFS production was found to be 5, and the incubation period of 72 hours was determined to be the most suitable for obtaining a high yield of CFS. Another aspect of the study aimed to identify multidrug-resistant P. aeruginosa isolates from burn
wound infections. The isolates were identified using the VITEK 2 system, and the phzM gene was detected in all nine strains. Furthermore, the study evaluated the effect of the CFS of the selected strain (E5) on the expression of the phzM gene. Results: The study showed a significant down-regulation of phzM gene expression in multidrug-resistant P. aeruginosa isolates following exposure to the CFS, indicating the potential of E. faecium as an effective antimicrobial agent against P. aeruginosa infections that are resistant to multiple drugs. We performed a primary screening to evaluate the effect of the CFS obtained from E. faecium (E5), and it was observed that the CFS showed a high inhibition zone of 23 mm against multidrug-resistant P. aeruginosa, as determined by the agar well diffusion assay. The study also investigated further to determine the optimal conditions for producing CFS. Conclusion: Down-regulation and up-regulation in the expression of the genes following exposure to CFS indicate the potential of E. faecium as an effective antimicrobial agent against multi-drug resistant (MDR) P. aeruginosa infections.
References
Nowakiewicz A, Zi’olkowska G, Tro’scia’nczyk A. et al. Determination of resistance and virulence genes in Enterococcus faecalis and E. faecium strains isolated from poultry and their genotypic characterization by ADSRRS-fingerprinting. Poult. Sci., 2016, 96, 986-996.
Yılmaz ES, Aslanta O, Onen SP, et al. Prevalence, antimicrobial resistance and virulence traits in enterococci from food of animal origin in Turkey. LWT Food Sci. Technol., 2016, 66, 20-26.
Leska A, Nowak A, Motyl I. Isolation and Some Basic Characteristics of Lactic Acid Bacteria from Honeybee (Apis mellifera L.) Environment A Preliminary Study. Agriculture 2022, 12, 1562.
Ahmed M E. The study of bacteriocin of Pseudomonas fluorescens and Citrus limon effects against Propionibacterium acnes and Staphylococcus epidermidis in acne patients. In Journal of Phys
ics: Conference Series (Vol. 1003, No. 1, p. 012004). IOP Publishing2008 https://doi.org/10.1088/1742-6596/1003/1/012004.
Qu J, Cai Z, Liu Y, et al. Persistent bacterial coinfection of a COVID-19 patient caused by a genetically adapted Pseudomonas aeruginosa chronic colonizer. Front. Cell. Infect. Microbiol., 2021, 11, 641920.
Shao X, Xie Y, Zhang Y, et al. Novel therapeutic strategies for treating Pseudomonas aeruginosa infection. Expert Opin. Drug Discov., 2020, 15, 1403-1423.
Gonçalves T, Vasconcelos U. Colour me blue: the history and the biotechnological potential of pyocyanin. Molecules, 2021, 26, 927. doi: 10.3390/ molecules26040927.
Carlsson M, Shukla S, Petersson AC. Pseudomonas aeruginosa in cystic fibrosis: pyocyanin negative strains are associated with BPI-ANCA and progressive lung disease. J Cyst Fibros., 2011, 10, 265-271.
Ahmed M E, Q Al-lam M, Abd Ali D. Evaluation of antimicrobial activity of plants extract against bacterial pathogens isolated from urinary tract infection among males patients. Al-Anbar Medical Journal, 2021, 17(1), 20-24.
Ahmed M E, Kadhim A. R. Alternative Preservatives of a “Nisin A” with Silver Nanoparticles for Bacteria Isolation from the Local Food Markets of Baghdad City. Prof.(Dr) RK Sharma, 2020. 20(4), 4975. https://doi.org/10.37506/mlu.v20i4.1946
Arimah BD, Ogunlowo O P. Identification of lactic acid bacteria isolated from Nigerian foods: medical importance and comparison of their bacteriocins activities. Journal of Natural Sciences Research, 2014, 4, 76-86.
Endo A, Salminen S. Honeybees and beehives are rich sources for fructophilic lactic acid bacteria. Systematic and applied microbiology. 2013, 36, 444-448.
Aureli P, Capurso L, Castellazzi Butler É, et al. A pilot study investigating lactic acid bacterial symbionts from the honeybee in inhibiting human chronic wound pathogens. Int. Wound J., 2016, 13(5), 729-737.
Olofsson TC, Vásquez A. Detection and identification of a novel lactic acid bacterial flora within the honey stomach of the honeybee Apis mellifera. Curr Microbiol 2008;57:356–63.
Ahmed ME, Al-Awadi AQ, Abbas AF. Focus of Synergistic Bacteriocin-Nanoparticles Enhancing Antimicrobial Activity Assay. Microbiological Journal. 2023 (6). 95-104. https://doi. org/10.15407/ microbiolj85.06.095
Seddiq SH, Zyara AM, Ahmed ME. Evaluation the Antimicrobial Action of Kiwifruit Zinc Oxide Nanoparticles Against Staphylococcus aureus Isolated from Cosmetics Tools. BioNanoScience. 2023, 1-10. https://doi.org/10.1007/s12668-023-01142
Kowalska JD, Nowak A, S´liz˙ewska K. et al. Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry. Pol. J. Microbiol. 2020, 69, 5-18.
Anwer A W, Saleh G M, Ahmed ME. Antimicrobial Susceptibility of Enterococcus faecium Isolated from Bee-gut on PhzM Gene of Pseudomonas aeruginosa Isolates from Infected Wounds. Ibn AL-Haitham Journal For Pure and Applied Sciences, 2024, 37(2), 41-50.
Ahmed ME, Abdul Muhsin ZA. Synergistic Effect of Gentamicin and Iron Oxide Nanoparticles on phzM Gene of Pseudomonas aeruginosa. Microbiological journal. 2024 (3). P. 2739. https://doi.org/10.15407/ microbiolj86.03.027
Faiq N H, Ahmed ME. Effect of Biosynthesized Zinc oxide Nanoparticles on Phenotypic and Genotypic Biofilm Formation of Proteus mirabilis. 2023. Baghdad Science Journal. https://doi.org/10.21123/bsj.2023.8067
Kasoob DS, Hummadi EH. Expression Of Rhlr gene in Pseudomonas aeruginosa affected by lactobacillus spp. journal of pharmaceutical negative results. 2022, 13, 508-512.
Tang H, Yang D, Zhu L, et al. Paeonol interferes with quorumsensing in pseudomonas aeruginosa and modulates inflammatory responses in vitro and in vivo. 2022. Frontiers in immunology, 13.
Elzeini HM, Ali AA, Nasr NF et al. Isolation and identification of lactic acid bacteria from intestinal tract of honey bee Apis Mellifera L. in Egypt. J Apic Res 2020; 59:1-10.
Se-Hyung K, Jung-Whan Ch, Hyo-Won J, et al. Identification And Phylogenetic Analysis Of Enterococcus Isolates Using Maldi-Tof Ms And Vitek 2. Original Article. (2023) 13:21.
Strateva T, Dimov SG, Atanasova D, et al. Molecular genetic study of potentially bacteriocinogenic clinical and
dairy Enterococcus spp. isolates from Bulgaria. Ann Microbiol 66, 381–387 (2016). https://doi.org/10.1007/
s13213-015-1120-3
Todorov S D. Diversity of bacteriocinogenic lactic acid bacteria isolated from boza, a cereal-based fermented beverage from Bulgaria, Food Control, 2010; 21(7),1011-1021, https:// doi.org/10.1016/j.foodcont.2009.12.020
Mahdi SM, Ahmed ME, Abbas A F, Effect of Enterococin – Zinc Oxide Nanoparticles on Gene Expression of rsbA Swarming Genes in Proteus mirabilis isolation Catheter Urine. Biomed Pharmacol J 2024;17(2).
Ahmed M, Alhammer A, Mohammed M, et al. Synergistic Effects Of Neem Oil And Gentamicin On Pseudomonas Aeruginosa Via Phzm Gene Downregulation: A Comprehensive Review. Journal of microbiology, biotechnology and food sciences.2024. DOI: 10.55251/jmbfs.11095
Barbosa AAT, Mantovani HC, Lopes DRG. and Santana, H. F. Like it acid and poor: a study of abiotic factors influencing streptococcus bovis hc5 growth and bacteriocin production. Journal of microbiology, biotechnology and food sciences, 2021, 421-426.
Ahmed, ME, Seddiq, SH. Effects Of Bacterocin From Mrsa And Pseudomonas Aeruginosa Against Biofilm Of Food Born Pathogen. Plant Archives, 2018, 18(2), 2770-2776. https:// doi.org/10.25258/ijpqa.v9i2.13647
Ahmed ME, Zahra MA, Ahmed Th. The Evolutionary Effects Of Bacillin And S-Pyocin Bacteriocin And Their Effects On Propionibacterium Acnes And Fungi. Biochem. Cell. Arch. 2020, 20, (Suppl 2), 3645-3649.
Salman M F, ME A.M. The effect of selenium nanoparticles on the expression of MexB gene of Pseudomonas aeruginosa Isolated from wound and burn infections. Iraqi JMS. 2024; 22 (1): 79-92. doi: 10.22578. IJMS, 22(10).
Ahmed ME, Al-awadi, a. Q. Enterococcus faecium bacteriocin efflux pump mexa gene and promote skin wound healing in mice. Journal of microbiology, biotechnology and food sciences, 2024, e10711-e10711.
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