Prostaglandin F2α disturbs oogenesis by causing meiotic spindle damage
DOI:
https://doi.org/10.2478/AMB-2024-0077Keywords:
prostaglandin F2 alpha, meiosis, oogenesis, oocytes, female infertility, meiotic spindleAbstract
According to recent data, prostaglandin F2 alpha can have negative influence on meiosis during oogenesis. Previously, we have found that this prostaglandin may accelerate in a dosage-dependent way the postovulatory ageing in ovulated mature oocytes, compromising the integrity of their meiotic spindles. Aim. The study aimed to investigate the effects of prostaglandin F2α on the course and outcome of oocyte meiosis in a mouse model. Materials and Methods. Mouse oocytes were matured in vitro in the presence of prostaglandin F2α in a concentration of 100 ng/ml. Their meiotic stage, spindle morphology and chromosome arrangement were assessed by immunofluorescent labeling of tubulin and fluorescent staining of DNA. Results. We obtained higher percentage of immature oocytes in metaphase I after the treatment than in untreated control oocytes. In addition, there were specific morphological changes in the meiotic spindles of oocytes exposed to prostaglandin F2α associated with a reduced number of fibers. Conclusion. It is probable that prostaglandin F2α has an impact on the microtubule dynamics of the meiotic spindle that can prevent the transition of maturing oocytes to the second meiotic division, most likely by triggering the spindle assembly checkpoint.
References
Duffy DM, Ko C, Jo M, et al. Ovulation: Parallels with Inflammatory Processes. Endocr Rev, 2019, 40(2):369-416. DOI: 10.1210/er.2018-00075
Nascimento BC, Ferreira CS, Oliveira SP, et al. Naproxen administration affects murine late folliculogenesis, reduces granulosa cell proliferation and the number of ovulated oocytes. Reprod Toxicol, 2023, 124:108527. DOI: 10.1016/j.reprotox.2023.108527
Boruszewska D, Kowalczyk-Zieba I, Suwik K, et al. Prostaglandin E2 affects in vitro maturation of bovine oocytes. Reprod Biol Endocrinol, 2020, 18(1):40. DOI: 10.1186/s12958-020-00598-9
Charpigny G, Marquant-Le Guienne B, Richard C, et al. PGE2 Supplementation of Oocyte Culture Media Improves the Developmental and Cryotolerance Performance of Bovine Blastocysts Derived from a Serum-Free in vitro Production System, Mirroring the Inner Cell Mass Transcriptome. Front Cell Dev Biol, 2021, 9:672948. DOI: 10.3389/fcell.2021.672948
Rodrigues SAD, Pontelo TP, Kussano NR, et al. Effects of Prostaglandins E2 and F2α on the in vitro maturation of bovine oocytes. Domest Anim Endocrinol, 2020, 72:106447. DOI: 10.1016/j.domaniend.2020.106447
Feng Y, Qi J, Xue X, et al. Follicular free fatty acid metabolic signatures and their effects on oocyte competence in non-obese PCOS patients. Reproduction, 2022, 164(1):1-8. DOI: 10.1530/REP-22-0023
Soto P, Natzke RP, Hansen PJ. Identification of possible mediators of embryonic mortality caused by mastitis: actions of lipopolysaccharide, prostaglandin F2alpha, and the nitric oxide generator, sodium nitroprusside dihydrate, on oocyte maturation and embryonic development in cattle. Am J Reprod Immunol, 2003, 50(3):263-272. DOI: 10.1034/j.1600-0897.2003.00085.x
Kolarov AI, Hadzhinesheva VP, Chakarova IV, et al. Prostaglandin F2α Causes Fast Degenerative Changes in Ovulated Mouse Oocytes. Folia Biol (Praha), 2021, 67(5-6):208-212.
Nikolova V, Delimitreva S, Chakarova I, et al. Dynamics of Lamins B and A/C and Nucleoporin Nup160 during Meiotic Maturation in Mouse Oocytes. Folia Biol (Praha), 2017, 63(1):6-12.
Markova M, Hadzhinesheva V, Zhivkova R, et al. Rearrangements of oocyte cytoskeleton during mammalian oogenesis. Acta Morphol Anthropol, 2016, 23:145-148.
Chakarova I, Delimitreva S, Hadzhinesheva V, et al. (2016). Correlation between defects of chromatin condensation and spindle assembly in oocytes of two mouse strains. CR Acad Bulg Sci, 2016, 69(9):1167-1174.
Hadzhinesheva V, Nikolova V, Chakarova I, et al. In vitro Maturing Mouse Oocytes Treated by Okadaic Acid – Effect on Cytoskeletal Structures and the Chromosome Spread. Acta Morphol Anthropol, 2015, 21:47-50.
Nikolova V, Markova M, Zhivkova R, et al. Karyosphere, the enigmatic “surrounded nucleolus” of maturing oocytes: review. Acta Morphol Anthropol, 2017, 24(1-2):78-84.
Lane S, Kauppi L. Meiotic spindle assembly checkpoint and aneuploidy in males versus females. Cell Mol Life Sci, 2019, 76(6):1135-1150. DOI: 10.1007/s00018-018-2986-6
López-Gatius F. Clinical prospects proposing an increase in the luteolytic dose of prostaglandin F2α in dairy cattle. J Reprod Dev, 2021, 67(1):1-3. DOI: 10.1262/jrd.2020-101
Thomas C, Cavazza T, Schuh M. Aneuploidy in human eggs: contributions of the meiotic spindle. Biochem Soc Trans, 2021, 49(1):107-118. doi: 10.1042/BST20200043
Wasielak-Politowska M, Kordowitzki P. Chromosome Segregation in the Oocyte: What Goes Wrong during Aging. Int J Mol Sci, 2022, 23(5):2880. doi: 10.3390/ijms23052880
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Journal Acta Medica Bulgarica 
