Assessment of enamel microroughness in primary and permanent teeth with initial carious lesions before and after acid application

Authors

DOI:

https://doi.org/10.2478/AMB-2025-0059

Keywords:

microroughness, profilometer, hydrochloric acid, orthophosphoric acid, primary teeth, permanent teeth, white spot lesions

Abstract

Aim: To evaluate the microroughness of primary and permanent teeth in the initial carious lesion (white spot) area before and after orthophosphoric and hydrochloric acid application. Material and Methods: The in vitro study included physiologically exfoliated primary molars and permanent third molars extracted for orthodontic reasons. The teeth were divided into eight groups according to the presence of initial caries lesions, type of dentition, the acid used, and the time of its application. The surface microroughness in the area of the carious lesion, both before and after etching, was assessed using a profilometer, and the results were compared. Carious lesions were etched either with 37% orthophosphoric acid for 30 seconds or with 15% hydrochloric acid for 120 seconds. Results: Higher microroughness values were found in the area of the initial carious lesion compared to the area with intact enamel for both dentitions. The microroughness of the enamel surface in the carious lesion area increased after applying orthophosphoric and hydrochloric acids in both primary and permanent teeth. The highest microroughness was recorded after conditioning with 15% hydrochloric acid. Microroughness data from carious primary and permanent teeth showed increased microporosity after etching with 37% orthophosphoric acid. Conclusion: Etching (with hydrochloric and orthophosphoric acid) of initial carious lesions of primary and permanent teeth significantly increases their microroughness.

References

Zawawi RN, Almosa NA. Assessment of enamel surface roughness and hardness with metal and ceramic orthodontic brackets using different etching and adhesive systems: An in vitro study. Saudi Dent J. 2023;35(6):641-650.

Beniash E, Stifler CA, Sun CY, et al. The hidden structure of human enamel. Nat Commun. 2019;10(1):4383.

Lacruz RS, Habelitz S, Wright JT, et al. Dental enamel formation and implications for oral health and disease. Physiol Rev. 2017 Jul 1;97(3):939-993.

Kunin AA, Evdokimova AY, Moiseeva NS. Age-related differences of tooth enamel morphochemistry in health and dental caries. EPMA J. 2015;6(1):3.

Xie Z, Yu L, Li S, et al. Comparison of therapies of white spot lesions: a systematic review and network meta-analysis. BMC Oral Health. 2023 Jun 1;23(1):346.

Malcangi G, Patano A, Morolla R, et al. Analysis of Dental Enamel Remineralization: A Systematic Review of Technique Comparisons. Bioengineering (Basel). 2023 Apr 12;10(4):472.

Roig-Vanaclocha A, Solá-Ruiz MF, Román-Rodríguez JL, et al. Dental Treatment of White Spots and a Description of the Technique and Digital Quantification of the Loss of Enamel Volume. Applied Sciences. 2020; 10(12):4369.

Lopes PC, Carvalho T, Gomes ATPC, et al. White spot lesions: diagnosis and treatment – a systematic review. BMC Oral Health. 2024 Jan 9;24(1):58.

Shan D, He Y, Gao M, et al. A comparison of resin infiltration and microabrasion for postorthodontic white spot lesion. Am. J Orthod. Dentofacial. Orthop. 2021; 160(4):516–522.

Wakwak МA, Alaggana NА, Morsy AS. Evaluation of surface roughness and microhardness of enamel white spot lesions treated by resin infiltration technique (icons): An In-vitro study. Tanta Dental Journal 2021; 18(3):88-91.

Weitman RJ, Eames WB. Plaque accumulation on composite surfaces after various finishing procedures. J Am Dent Assoc. 1975;65:29–33.

Teutle-Coyotecatl B, Contreras-Bulnes R, Rodríguez-Vilchis LE, et al. Effect of Surface Roughness of Deciduous and Permanent Tooth Enamel on Bacterial Adhesion. Microorganisms. 2022 Aug 24;10(9):1701.

Detara M, Triaminingsih S, Irawan B. Effects of nano calcium carbonate and siwak toothpaste on demineralized enamel surface roughness, J Phys Conf Ser 2018, 1073 (3): 032011.

Arnold WH, Meyer AK, Naumova EA. Surface roughness of initial enamel caries lesions in human teeth after resin infiltration. Open Dent J 2016; 10(1): 505-15.

Chabuk MM, Al-Shamma AM. Surface roughness and microhardness of enamel white spot lesions treated with different treatment methods. Heliyon. 2023 Jul 18;9(7):e18283.

Erdur EA, Akın M, Cime L, et al. Evaluation of Enamel Surface Roughness after Various Finishing Techniques for Debonding of Orthodontic Brackets. Turk J Orthod. 2016 Mar;29(1):1-5.

Muthuvel P, Ganapathy A, Subramaniam MK, et al. Erosion Infiltration Technique’: A Novel Alternative for Masking Enamel White Spot Lesion. J Pharm Bioallied Sci. 2017 Nov;9(Suppl 1):S289-S291.

Jani B, Shah A, Shankar C, et al. Effectiveness of Different Etching Agents on Enamel Surface and Shear Bond Strength: An In Vitro Evaluation. Cureus. 2024 Feb 11;16(2):e54008.

Ersahan S, Alakus Sabuncuoglu F. Effect of surface treatment on enamel surface roughness. J Istanb Univ Fac Dent. 2016 Jan 12;50(1):1-8.

Nevárez-Rascón M, Molina-Frechero N, Adame E, et al. Effectiveness of a microabrasion technique using 16% HCL with manual application on fluorotic teeth: A series of studies. World J Clin Cases. 2020 Feb 26;8(4):743-756.

Pini NI, Sundfeld-Neto D, Aguiar FH, et al. Sundfeld RH, Martins LR, Lovadino JR, Lima DA. Enamel microabrasion: An overview of clinical and scientific considerations. World J Clin Cases. 2015;3:34–41.

Kannan A, Padmanabhan S. Comparative evaluation of Icon® resin infiltration and Clinpro™ XT varnish on colour and fluorescence changes of white spot lesions: a randomized controlled trial. Prog Orthod. 2019 Jun 17;20(1):23.

Yazkan B, Ermis RB. Effect of resin infiltration and microabrasion on the microhardness, surface roughness and morphology of incipient carious lesions. Acta Odontol Scand. 2018 Oct;76(7):473-481

Downloads

Published

02.09.2025

Issue

Section

ORIGINAL ARTICLES

How to Cite

Bogovska-Gigova, R., Hristov, K., Gateva, N., & Angelova, L. (2025). Assessment of enamel microroughness in primary and permanent teeth with initial carious lesions before and after acid application. Acta Medica Bulgarica, 52(3), 38-43. https://doi.org/10.2478/AMB-2025-0059